Figure 6

Incomplete autophagy promotes the proliferation of M. hyopneumoniae in 3D4/21 cells. A Western blotting of LC3-II, p62, and P97 after 3D4/21 cells were pretreated with 3-MA and then infected with AH at an MOI of 2. B Western blotting of LC3-II, p62, and P97 after 3D4/21 cells were pretreated with 3-MA and then infected with AH at an MOI of 200. C Effect of 3-MA on the reproduction of AH in 3D4/21 cells at different MOIs. D Western blotting of LC3-II, p62, and P97 after infecting 3D4/21 cells with AH at an MOI of 2 and treating them with different autophagy regulators for 6 h. E Western blotting of LC3-II, p62, and P97 after infecting 3D4/21 cells with AH at an MOI of 200 and treating them with different autophagy regulators for 6 h. F Effects of different autophagy regulators on the proliferation of intracellular AH in 3D4/21 cells at an MOI of 2 and an MOI of 200. Mean ± SD values from 3 independent experiments were compared using a t test (A, B, C) or one-way ANOVA (D, E, F); *p ≤ 0.05, **p ≤ 0.01.