Figure 3

Accumulation of LC3 after treatment with different autophagy inhibitors in 3D4/21 cells with or without infection by the AH strain. A Western blotting of LC3-II in AH-infected cells and mock-infected cells after treatment with HCQ for different durations. B Western blotting of LC3-II in AH-infected cells and mock-infected cells after treatment with BAF for different durations. C Immunofluorescence of LC3 puncta (red) in AH-infected and uninfected 3D4/21 cells that were treated with HCQ for 6 h. D Immunofluorescence of LC3 puncta (red) in AH-infected and uninfected 3D4/21 cells that were treated with BAF for 6 h. Mean values from 3 independent experiments are presented in A and B; 35 cells from each treatment were counted to generate the data shown in C and D. Statistical significance was determined using one-way ANOVA (A, B) or a t test (C, D). ns: not significant, *p ≤ 0.05, **p ≤ 0.01.