Table 1 Primary primers used in this study.
Primer name | Sequence (5′–3′) |
|---|---|
Nsp4-F | CGGGGTACCATGGGTGCTTTCAGAACTCAAAAGC |
Nsp4-R | CCGCTCGAGTTATTCCAGTTCGGGTTTGGCAGCAAGC |
pGBP1-F | CCCTCGAGATGGATTACAAGGATGACGACGATAAGATGGCCTCAAAGGTGCACAT |
pGBP1-R | CGGGATCCTTAGCTCAGGAAACATTCTT |
pGBP11-290-F | CCCTCGAGATGGATTACAAGGATGACGACGATAAGATGGCCTCAAAGG TGCACAT |
pGBP11-290-R | CGGGATCCTTACAGACGGGGCCCGGTGACC |
pGBP1288-591-F | CCCTCGAGATGGATTACAAGGATGACGACGATAAGCCCCGTCTGGAGACCCTGGTGC |
pGBP1288–591-R | CGGGATCCTTAGCTCAGGAAACATTCTT |
pGBP1-K51A-F1 | CCCTCGAGATGGATTACAAGGATGACGACGATAAGATGGCCTCAAAGG TGCACAT |
pGBP1-K51A-R1 | CATCAGGTAGGATGCGCCTGTGCGGTAC |
pGBP1-K51A-F2 | GTACCGCACAGGCGCATCCTACCTGATGAAC |
pGBP1-K51A-R2 | CGGGATCCTTAGCTCAGGAAACATTCTT |
pGBP1-R48A-F1 | CCCTCGAGATGGATTACAAGGATGACGACGATAAGATGGCCTCAAAGGTGCACAT |
pGBP1-R48A-R1 | GATTTGCCTGTTGCGTACAGGCCCAC |
pGBP1-R48A-F2 | GTGGGCCTGTACGCAACAGGCAAATC |
pGBP1-R48A-R2 | CGGGATCCTTAGCTCAGGAAACATTCTT |
pGBP1-ΔCAAX-F | CCCTCGAGATGGATTACAAGGATGACGACGATAAGATGGCCTCAAAGG TGCACAT |
pGBP1-ΔCAAX-R | CGGGATCCTTACTTTGATTTTTTCATCTTCTTC |
ORF7-qF | AGATCATCATCGCCCAACAAAAC |
ORF7-qR | GACACAATTGCCGCTCACTA |
pGBP1-qF | AAGTCAGCCGAGTCCTCAATCC |
pGBP1-qR | GCAGCACCTTCGTCTACAACAG |
β-actin-qF | TCCCTGGAGAAGAGCTACGA |
β-actin-qR | AGCACTGTGTTGGCGTACAG |