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Figure 5 | Veterinary Research

Figure 5

From: Porcine reproductive and respiratory syndrome virus non-structural protein 4 cleaves guanylate-binding protein 1 via its cysteine proteinase activity to antagonize GBP1 antiviral effect

Figure 5

Screening of PRRSV proteins that interact with GBP1. A Western blotting identification of the expression of PRRSV-relevant proteins. HEK293T cells were transfected with the plasmids expressing HA-tagged proteins of PRRSV. The cells were harvested at 36 h post-transfection and subjected to Western blotting analysis. B Screening of PRRSV proteins that interact with GBP1. HEK293T cells were co-transfected with the plasmids expressing HA-tagged proteins of PRRSV and Flag-GBP1 for 48 h. Cell samples were lysed and co-immunoprecipitation was performed using anti-flag pAb. GBP1, guanylate-binding protein 1; PRRSV, porcine reproductive and respiratory syndrome virus.

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Veterinary Research

ISSN: 1297-9716