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Figure 4 | Veterinary Research

Figure 4

From: Porcine reproductive and respiratory syndrome virus non-structural protein 4 cleaves guanylate-binding protein 1 via its cysteine proteinase activity to antagonize GBP1 antiviral effect

Figure 4

GTPase activity and dimerization of GBP1 is necessary for the inhibition of PRRSV replication. A Map of the full-length and three site-specific mutants of GBP1. B Western blotting verification of Marc-145 cells stably expressing GBP1-ΔCAAX, -K51A, -R48A using rabbit anti-flag pAb. Marc-145-Vector, -GBP1-flag, -GBP1-ΔCAAX-flag, -GBP1-K51A-flag or -GBP1-R48A-flag were infected with GFP-PRRSV at a MOI of 0.1. At 36 h post-infection, cells were harvested for the C detection of N protein expression via Western blotting (C), and D for GFP-PRRSV-positive cell analysis via flow cytometry. E Marc-145-GBP1-flag, -GBP-ΔCAAX-flag, -GBP1-K51A-flag or -GBP1-R48A-flag were used for the detection of GBP1 using anti-flag pAb. Actin was visualized with fluorescently labeled phalloidin. GBP1, guanylate-binding protein 1; PRRSV, porcine reproductive and respiratory syndrome virus; GFP, green fluorescent protein.

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