Table 1 Primers for Q–RT–PCR analysis of gene expression.
Target gene | Forward (5'-3') | Reverse (5'-3') |
|---|---|---|
AIF | AGGCTGACACTCTTCCG | TCTGTGGTCCAGTTGCTC |
XIAP | AGGCACTGACTATGACC | TCACTTTACAGCCTTCG |
Apaf-1 | AGAGGGCACAAGGAAGCTATCA | AACTTACTACCATCAGGCGAAACA |
Smac | AGTCTGGCAGGTGGTG | CGTAACGCTGTCATCC |
Bak | TCACCAAGGAGAATGCCTACGA | GCCTGTTATGCCGTGCTGGTA |
Bcl-xL | AAAATGTCCAGCGGCAACC | AAGCCAACTCAGTCCTGTTCTCA |
PARP | CTGGACTAAGTGCGTTGC | AGCCTCTGGAGGGAATA |
Bid | CTGTCGCAGGCTGTGGAAGT | AGACACGCTGGAGAAGGAAGG |
Cyt C | TGAGGATACCCTGATGGAGTACTTG | CTTCGCAGTGGCATCTTTCAG |
FasL | AGCAGCCAGATGTTGAGCCA | GTCCCTCTCCCTGAGCTTGAATA |
Fas | TATCTGCACTGACTTCAAGCGTATT | TGTTGGCTGTTGCATGACTGG |
TNFSF10 | CCCTGACACTTCCACG | CGATTCCCAGTTGCTTA |
TNFRSF10B β-actin | TGCGAGATGTGCCAGAAGT GATCACAGCCCTGGCACC | GGCGAAGACGTGGAAAGA CGGATTCATCATACTCCTGCTT |