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Table 1 BSP Primer sequences for methylation analysis of the chTERT amplicon

From: The expression level of chicken telomerase reverse transcriptase in tumors induced by ALV-J is positively correlated with methylation and mutation of its promoter region

Primers position (bp)1

Sequences (5′-3′)

Annealing temperature

Product (bp)

−579-F2

TTTTTTTTATTAAATTGTGTTATTG

65 ℃

194

−386-R3

CTCTTACTTTATCCCTAAAAAAACC

−315-F

AGTTTAATTGTTAATTTATTTTTATTT

64 ℃

159

−157-R

TTAAATTTAAAAACAATTTCTTCTC

−167-F

TTAAATTTAATTTGAGTTTTTTTTAG

69 ℃

323

 + 156-R

ATACCACCCTCCTACAACC

 + 263-F

TTTGTTTTTAGTAGGTAGGGAGGAG

67 ℃

267

 + 529-R

AACCCCAAACATACAAATCTTTAAC

 + 576-F

TTGGGAGGGAAGTATTATTTTTTTT

67 ℃

264

 + 869-R

CCCTTTTAATCCTACTCCTCATACC

  1. 1The primer position is relative to the start codon ATG, and upstream of the start codon is marked as “−”, while ATG and its downstream regions are marked as “ + ”
  2. 2“F” stands for upstream primer
  3. 3“R” stands for downstream primer