Skip to main content
Figure 6 | Veterinary Research

Figure 6

From: Molecular characterization and determination of the biochemical properties of cathepsin L of Trichinella spiralis

Figure 6

Identification of rTsCatL2. A SDS–PAGE. Lane 1: recombinant bacterial pMAL-c2x-TsCatL2 lysate before induction; Lane 2: pMAL-c2x-TsCataL2 lysate after 1 mM IPTG induction at 25 °C; Lane 3: purified rTsCatL2 with MBP. B Western blotting analysis of rTsCatL2 antigenicity. Lane 1: Serum of mice infected with T. spiralis; Lane 2: anti-rTsCatL2 serum; Lane 3: serum of normal mice. C Analysis of autocatalytic cleavage of rTsCatL2 at 37 °C for 30 min. Lane 1: pH 7.0; Lane 2: pH 5.0. Mature-TsCatL is indicated with a red arrow.

Back to article page

Veterinary Research

ISSN: 1297-9716