Figure 10

Enzymatic characteristics of rTsCatL2. A Different temperatures. B Different pH values at 55 °C. C Treatments with different metal ions. D Different inhibitors and activators. The concentration of E64 was 10 μM, and PMSF, AEBSF, 1,10-phenanthroline, pepstatin A, EDTA, L-cysteine and DTT were used all at 1 mM. E Michaelis–Menten curve and Lineweaver–Burk plot of rTsCatL2 at pH 4.5, 37 °C and 5 mM DTT. F Michaelis–Menten curve and Lineweaver–Burk plot of rTsCatL2 at pH 4.5, 55 °C and 5 mM DTT.