Table 2 Primers used in this study.
Primers | Sequences (5′–3′) |
|---|---|
orf1B-for | ATGGTACCCGCCTGGGCATCTTCTC |
orf1B-int-rev | GCGTAGCTGCGTCAGCATGG |
orf1B-int-for | GCTGACGCAGCTACGCCTGGAGGAGAGCGAATGA |
orf1B-rev | ATGGTACCGCAGCCACGCATTCAGC |
orf1B-com-for | CACTGTCCGACCGCTTTGGCCGGGTGGGCGAATACCGAGC |
orf1B-com-int-rev | ATATCGCGTAGCTGCGTCAGAGCGTAATCTGGAACATCGTATGGGTACATGGCTGGCCT |
orf1B-com-int-for | AGGCCAGCCATGTACCCATACGATGTTCCAGATTACGCTCTGACGCAGCTACGCGATAT |
orf1B-com-rev | GCCAAAGCGGTCGGACAGTGCTCCGAGA |
pACYC184-for | GGCCTCAACCTACTACTGGGCTGCTTCC |
pACYC184-rev RT-16S-for RT-16S-rev RT-eseJ-for RT-eseJ-rev | GCCAAAGCGGTCGGACAGTGCTCCGAGA ACTGAGACACGGTCCAGACTCCTAC TTAACGTTCACACCTTCCTCCCTAC CTGCTGGCGACCTTTTATCC AGTAGTCCGGATTGCGCTTA |
esaN::3 × FLAG-for | GCCACCCTCAGCGCACTGTACAGCGCCGTAGGCCAGCCATGCGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAG |
esaN::3 × FLAG-rev | CTGGAGCTGCTGCTGTCGTCGCCGTACGCCGTCAGCCTCATC |
eseE::3 × FLAG-for | ATCGTCTGGATGATGCGCTGTGGCTGCGCTATTGCTGCCGCGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAG |
eseE::3 × FLAG-rev | GATATATTATTACATTATCAGGGCGTGCGCCCCGGGGCTTTGGAAACATATGAATATCCTCCTTAGT |