Table 1 Bacterial strains and plasmids used in this study.

Strains

 E. piscicida

  WT

PPD130/91, Km^s, Col^r, Amp^s

[25]

  ΔesaN

In-frame deletion of aa 21–421, PPD130/91

[10]

  Δorf1B

In-frame deletion of aa 7–117, PPD130/91

This study

  WT/pACYC184

WT strain transformed with pACYC184

This study

  Δorf1B/pACYC184

Δorf1B strain transformed with pACYC184

This study

  Δorf1B/orf1B

Δorf1B with pACYC184-HA-orf1B

This study

  WT esaN::3 × FLAG

PPD130/91, with chromosomal expression of EsaN-3 × FLAG, Amp^r, Km^r

This study

  WT esaN::3 × FLAG/orf1B

WT esaN::3 × FLAG with pACYC184-HA-orf1B

This study

  WT eseE::3 × FLAG

PPD130/91, with chromosomal expression of EseE-3 × FLAG, Amp^r, Km^r

This study

  WT eseE::3 × FLAG/orf1B

WT eseE::3 × FLAG with pACYC184-HA-orf1B

This study

Plasmids

 pACYC184

Tet^r, Cm^r

Amersham

 pACYC184-HA-orf1B

pACYC184 with HA-orf1B

This study

 pRE112

pGP704 suicide plasmid, pir dependent; Cm^r oriT, oriV, sacB

[27]

 pKD46

Red helper plasmid, Amp^r

[30]

 pKD4

Template plasmid with FLP recognition target site, Km^r

[30]