Figure 7

Effects of rHcGATA on Th9-cell proliferation and IL-9 transcription. A Agarose gel electrophoresis of the HcGATA gene. Lane 1: reverse transcription PCR products of HcGATA; Lane M: DNA molecular weight marker. B Purification of rHcGATA. Lane 2: rHcGATA before purification. Lane 3: purified rHcGATA. Lane M: standard protein molecular marker. C Western blot. Lane 4: rHcGATA protein recognized by serum from an H. contortus-infected goat. Lane 5: rHcGATA was not recognized by normal serum. Lane M: standard protein molecular marker. D The effects of HcGATA on the proliferation of Th9 cells in vitro. PBMC-derived Th9 cells treated with a control (0 μg/mL) or different concentrations of HcGATA (5, 10, 20, 40, 60 μg/mL) were tested by flow cytometry using antibodies specific for typical intracellular cytokines (IL-9 and IL-10). E Proportions of Th9 cells observed with different concentrations of HcGATA (0, 5, 10, 20, 40 and 60 μg/mL). Data are presented as the mean ± SD representative of triplicate experiments (ns p > 0.05, *p < 0.05, ****p < 0.0001). F Fold change in relative IL-9 mRNA expression. Goat PBMCs were stimulated with different concentrations of rHcGATA. The significance level was set at ***p < 0.001, or ****p < 0.0001, and “ns” indicates non-significance compared with the control group. Data are representative of three independent experiments.