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Figure 3 | Veterinary Research

Figure 3

From: Identification of excretory and secretory proteins from Haemonchus contortus inducing a Th9 immune response in goats

Figure 3

Co-IP assays indicated that HcESPs bind to Th9 cells. Lane M: marker. Lanes 1 and 2: SDS–PAGE showing that cell lysates were immunoprecipitated by anti-HcESPs rat IgG (Lane 1) or normal rat IgG as a negative control (Lane 2). Lanes 3 and 4: Western blot showing that cell lysates were immunoprecipitated by anti-HcESPs rat IgG (Lane 4) or normal rat IgG as a negative control (Lane 4). Cell lysates from in vitro experiments (A) or in vivo experiments collected at 7 (B), 15 (C), 35 (D) and 50 (E) dpi. Anti-ESPs rat IgG was used in the Western blot analysis to identify proteins that bound to Th9 cells.

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