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Figure 2 | Veterinary Research

Figure 2

From: Identification of excretory and secretory proteins from Haemonchus contortus inducing a Th9 immune response in goats

Figure 2

Th9-cell sorting by flow cytometry. A Th9 cells induced by HcESPs in vitro. PBMCs were treated with PBS (0 μg/mL HcESPs as the control) (A Panel 1) or 80 μg/mL HcESPs (A Panel 2). Th9 cells were sorted by flow cytometry using CD2 + CD4 + IL-9 + IL-10 + as the gate. B–E Percentages of Th9 cells at 7 (B), 15 (C), 35 (D) and 50 (E) dpi. Samples were collected from the blank group (B–E Panel 1) and challenge group (B–E Panel 2) at 7, 15, 35 and 50 dpi.

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