Table 1 Plasmids used in this study
Plasmids | Genotype/Description | Source |
|---|---|---|
pRS416-GAL1 | GAL1 promoter, 3 × Flag tag, URA3, ampicillin | [15] |
pRS416-GAL1-LI0666 | GAL1 promoter, 3 × Flag-LI0666, URA3, ampicillin | This study |
pRS416-GAL1-LI0666Y149/186A | GAL1 promoter, 3 × Flag-LI0666Y149/186A, URA3, ampicillin | This study |
pYES2URA-RipI | 2 µ, GAL1/GAL10, RipI, URA3 | [23] |
pBAD24-YopE-HA | araC promoter, YopE-HA, ampicillin | This study |
pBAD24-RplJ-HA | araC promoter, RplJ-HA, ampicillin | This study |
pBAD24-LI0666-HA | araC promoter, LI0666-HA, ampicillin | This study |
pBAD24-LI0666N40-YopE∆N15-HA | araC promoter, LI0666N40-YopE∆N15-HA, ampicillin | This study |
pYES2URA-EGFP | EGFP gene fragment was inserted into pYES2URA under GAL1 promoter | This study |
pYES2URA-LI0666-EGFP | LI0666 gene fragment was inserted into pYES2URA-EGFP under GAL1 promoter | This study |
pYES2URA-LI0666Y149A-EGFP | LI0666 harboring A in place of Y at position 149 | This study |
pYES2URA-LI0666Y186A-EGFP | LI0666 harboring A in place of Y at position 186 | This study |
pYES2URA-LI0666Y149/186A-EGFP | LI0666 harboring A in place of Y at position 149 and 186 | This study |
pYES2URA-LI0666△N39-EGFP | LI0666 harboring deletion for 1–39 | This study |
pYES2URA-LI0666△C64-EGFP | LI0666 harboring deletion for 141–204 | This study |
pEGFP-C1 | CMV, Neomycin, kanamycin, EGFP | Clontech |
pEGFP-C1-LI0666Y149/186A | LI0666149/186A gene fragment was inserted into pEGFP-C1 in frame with EGFP at the N-terminal | This study |
pEGFP-C1-LI0666 | LI0666 gene fragment was inserted into pEGFP-C1 in frame with EGFP at the N-terminal | This study |
pEGFP-C1-LI0666△C64 | LI0666 harboring deletion for 141–204 was inserted into pEGFP-C1 in frame with EGFP at the N-terminal | This study |