Figure 4

Subcellular localization of GFP-LI0666 fusion proteins in S. cerevisiae and mammalian cells. A Cells were cultured for 10 h under inducting conditions in galactose medium at 30 °C. Empty vector (pYES2/NTA encoding EGFP) was used as the negative control. LI0666-EGFP, LI0666^Y149/186A-EGFP, LI0666_ΔN39-EGFP and LI0666_ΔC64-EGFP, which is a deletion mutant of LI0666 lacking the C-terminal 64 amino acids, was expressed in yeast respectively. B EGFP-LI0666, EGFP-LI0666^Y149/186A and EGFP-LI0666_ΔC64 were transfected into HEK293T cells. After incubation at 37 °C for 24 h, the nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole), and fluorescence was observed using a fluorescence microscope.