Figure 3From: Lawsonia intracellularis LI0666 is a new EPIYA effector exported by the Yersinia enterocolitica type III secretion systemLI0666 tyrosine phosphorylation in mammalian cells. EGFP-LI0666 and EGFP-LI0666Y149/186A were transfected into HEK293T (A), HeLa (B), and IPEC-J2 (C) cells. Cell lysates were subjected to Western blot using an anti-GFP antibody and a 4G10 antibody. Lysate from cells containing empty vector was used as the negative control.Back to article page