Figure 2

LI0666 expression modulates yeast growth. A LI0666 expression inhibits yeast growth. W303-1A and BY4741 yeast strains carrying the yeast expression vector pRS416-GAL1, either empty or encoding LI0666 or LI0666^Y149/186A with a N-terminal 3 × Flag, were grown overnight in repressing medium (2% glucose). Cultures were then normalized to OD₆₀₀ 1.0, and serial tenfold dilutions were grown at 30 °C for 2 and 3 days in repressing and inducing medium (2% galactose and 1% raffinose), respectively. W303-1A yeast carrying the yeast expression vector pYES2/NT-RipI was used as the positive control. B Induction of expression was verified by Western blotting using an anti-Flag antibody and an anti-phosphotyrosine antibody (4G10) for 3 × Flag-LI0666 and 3 × Flag-LI0666^Y149/186A.