Figure 3
From: ASFV pD345L protein negatively regulates NF-κB signalling by inhibiting IKK kinase activity
pD345L suppresses NF-κB activation at or downstream of IKKα/β and upstream of p65. 293 T cells were transfected with NF-κB-Luc (200 ng), pCMV-RL (2 ng), pcDNA3-Flag-TRAF6 (200 ng) (A), pcDNA4-HA-TAK1/TAB1 (each 200 ng) (B), pcDNA4-HA-TBK1 (C), pcDNA4-HA-IKKα (200 ng) (D), pcDNA4-HA-IKKβ (200 ng) (E), pcDNA4-HA-p65 (100 ng) (F), p3XFLAG-CMV-D345L (200 ng) or empty vector (corresponding quantity). The cells were collected 24 h post-transfection, and luciferase activity was measured.