Figure 1
From: ASFV pD345L protein negatively regulates NF-κB signalling by inhibiting IKK kinase activity
pD345L inhibits IFNβ expression through cGAS-STING-mediated NF-κB signalling. A 293 T cells transfected with pGL3-Basic-IFNβ-Luc and pCMV-RL along with empty vector, pcDNA3.1-HA-cGAS, pcDNA3-2xFlag-STING and/or p3XFLAG-CMV-D345L for 24 h were collected to measure luciferase activities. B The experiment was performed as shown in A except using an increasing amount of p3XFLAG-CMV-D345L. C 293 T cells transfected with pGL3-Basic-IFNβ-Luc and pCMV-RL along with empty vector, pcDNA3.1-HA-cGAS, pcDNA3-2xFlag-STING and/or p3XFLAG-CMV-D345L, p3XFLAG-CMV-MGF-505-7R for 24 h were collected for measuring luciferase activities. D The experiment was performed as shown in A except using NF-κB-Luc. E The experiment was performed as shown in A except using IRF3-Luc. F The experiment was performed with 293 T cells transfected with pGL3- Basic-IFNβ-Luc, pCMV-RL and p3XFLAG-CMV-D345L or empty vector for 24 h followed by treatment with TNFα (40 ng/mL) for 6 h. The “-” symbols represent empty vectors for the corresponding coding plasmids.