Table 1 Strains and plasmids used in this study
Strains or plasmids | Description | Reference |
|---|---|---|
Strains | ||
APEC40 | wild-type strain, isolated from sick chicken | This study |
APEC40-△yqeH | yqeH deletion mutant in APEC40 | This study |
APEC40-C△yqeH | APEC40-△yqeH with plasmid pSTV28-yqeH | This study |
DH5a | F-,△(lacZYA-argF)U169,recA1,endA1,hsdR17 (rk-,mk +), phoA,supE44, γ- | TIANGEN |
Plasmids | ||
pKD46 | Amp; expresses γ red recombinase | This study |
pKD3 | Cm gene, template plasmid | This study |
pCP20 | Cm, Amp, yeast Flp recombinase gene, FLP | This study |
pSTV28 | Cm, lacZ | Takara |
pSTV28-yqeH | pSTV28 derivative harboring yqeH gene | This study |