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Figure 1 | Veterinary Research

Figure 1

From: YqeH contributes to avian pathogenic Escherichia coli pathogenicity by regulating motility, biofilm formation, and virulence

Figure 1

PCR analysis of mutant strain APEC40-ΔyqeH and complemented strain APEC40-CΔyqeH. A Schematic diagram of the strategy used to construct the APEC40 yqeH deletion mutant. The yqeH gene was deleted by replacing the partial gene sequence with a chloramphenicol-resistance cassette. The primers used for confirmation of the yqeH deletion are indicated. B Confirmation of the yqeH mutant strain APEC40-ΔyqeH. Lane identities in (B)—M: 2000-bp DNA marker; 1–2: identification of wild-type APEC40-yqeH strain (lane 1, 252 bp; lane 2, 2256 bp); 3–4: identification of APEC40-ΔyqeH-Cm (lane 3, 0 bp; lane 4, 2637 bp); 5–6: identification of APEC40-ΔyqeH (lane 5, 0 bp; lane 6, 1623 bp). C Confirmation of the complemented strain APEC40-CΔyqeH. Lane identities in (C)—M: 2000-bp DNA marker; 1: Recombinant plasmid pSTV28 blank control identification, 193 bp; 2: APEC40-CΔyqeH identification, 826 bp.

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