Figure 1

PPV infection suppresses mTOR phosphorylation. PTCs were infected with PPV at MOI = 1 or were mock infected for 0 h, 6 h, 12 h, and 24 h. A Cell lysates were collected individually for Western blotting analysis with anti-mTOR, anti-p-mTOR, anti-Beclin1, anti-LC3A/B and anti-β actin antibodies. B–D Ratios of p-mTOR to mTOR, as well as Beclin1 and LC3 II to β-actin, as reported in A, were calculated and analysed. E, F The mRNA levels of beclin1 and lc3 were detected by quantitative-polymerase chain reaction (q-PCR). The results are the mean ± SEM (SD) of three experiments, * p < 0.05; ** p < 0.01.