PCR conditions and efficiency | |||||||
---|---|---|---|---|---|---|---|
Cytokine | Initial Denaturation | Denaturation | Annealing | Elongation | Number of PCR cycles | PCR efficiency | R |
TNFα | 95 °C (10 min) | 95 °C (15 s) | 60 °C (1 min) | 40 | 2.168 | 0.994 | |
IFNγ | 95 °C (10 min) | 95 °C (15 s) | 60 °C (1 min) | 40 | 1.982 | 0.999 | |
CCL28 | 95 °C (3 min) | 95 °C (15 s) | 56 °C (30 s) | 60 °C (30 s) | 45 | 2.097 | 0.997 |
IL-1α | 94 °C (2 min) | 94 °C (20 s) | 60 °C (1 min) | 45 | 1.983 | 0.999 | |
IL-8 | 94 °C (2 min) | 94 °C (20 s) | 60 °C (1 min) | 45 | 1.992 | 0.998 | |
Cyclophilin | 94 °C (3 min) | 94 °C (45 s) | 54 °C (45 s) | 60 °C (45 s) | 30 | 1.864 | 0.999 |