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Table 4 Quantitative PCR conditions and efficiency, R values and number of cycles used for each porcine cytokine and cyclophilin cDNA

From: Nonspecific protection of heat-inactivated Mycobacterium bovis against Salmonella Choleraesuis infection in pigs

PCR conditions and efficiency

Cytokine

Initial Denaturation

Denaturation

Annealing

Elongation

Number of PCR cycles

PCR efficiency

R

TNFα

95 °C (10 min)

95 °C (15 s)

60 °C (1 min)

40

2.168

0.994

IFNγ

95 °C (10 min)

95 °C (15 s)

60 °C (1 min)

40

1.982

0.999

CCL28

95 °C (3 min)

95 °C (15 s)

56 °C (30 s)

60 °C (30 s)

45

2.097

0.997

IL-1α

94 °C (2 min)

94 °C (20 s)

60 °C (1 min)

45

1.983

0.999

IL-8

94 °C (2 min)

94 °C (20 s)

60 °C (1 min)

45

1.992

0.998

Cyclophilin

94 °C (3 min)

94 °C (45 s)

54 °C (45 s)

60 °C (45 s)

30

1.864

0.999

  1. CCL28, C–C Motif chemokine ligand 28; IFNγ, Interferon gamma; IL-1α, Interleukin 1 alfa; IL-8, Interleukin 8; TNFα, Tumour necrosis factor alpha.