Table 3 Details of primer sequences for RT-PCR assessment of pulmonary cytokines in pigs challenged with S. Choleraesuis
Target | Amplicon size (base pairs) | Primer Forward (5ʹ–3ʹ) | Primer Reverse (5ʹ–3ʹ) | References |
|---|---|---|---|---|
TNFα | 134 | ACTCGGAACCTCATGGACAG | AGGGGTGAGTCAGTGTGACC | [76] |
IFNγ | 79 | TGGTAGCTCTGGGAAACTGAATG | GGCTTTGCGCTGGATCTG | [76] |
CCL28 | 145 | GCTGCTGCACTGAGGTTTC | TGAGGGCTGACACAGATTC | [68] |
IL-1α | 274 | GCTGCTGTGCTAAATAACCT | CTTGTGGCAATAAACAACTTT | [77] |
IL-8 | 240 | TCTTGGCAGTTTTCCTGCTT | CAACCTTCTGCACCCACTTT | [77] |
Cyclophilin | 369 | TAACCCCACCGTCTTCTT | TGCCATCCAACCACTCAG | [69] |