Figure 4
From: Functional characterization of goose IRF1 in IFN induction and anti-NDV infection
Goose IRF1 is involved in the regulation of IFNs, proinflammatory cytokines, and ISGs. A DF-1 cells were cotransfected with chIFN-β luciferase reporter plasmids (pRL-TK and pGL-chIFN-β-Luc) and with pcDNA3.1-Flag or pcDNA3.1-GoIRF1-Flag. Luciferase assays were performed 24 h after cotransfection. B GoIRF1 dose-independently induced IFN-β induction. C GEFs were cotransfected with chIFN-β luciferase reporter plasmids and with pcDNA3.1-Flag or pcDNA3.1-GoIRF1-Flag. Luciferase assays were performed 24 h after cotransfection. D Endogenous mRNA levels of goose IFNs (IFN-α, IFN-κ, IFN-γ, and IFN-λ) after stimulation with GoIRF1 or an empty vector. E Relative mRNA levels of proinflammatory cytokines (IL-6 and IL-8) after transfection with GoIRF1 or an empty vector. F Relative mRNA levels of ISGs (PKR, OASL, and Mx-1) after transfection with GoIRF1 or an empty vector. Error bars represent standard deviations. The difference between the experimental and control groups was *p < 0.05, **p < 0.01 or ***p < 0.001.