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Table 7 Cross-protection of NSLC-PLGA nanospheres against four Eimeria spp.

From: A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens

Challenged Eimeria spp.

Group

Coefficient of growth (14–28 days of age)

Coefficient of growth (28–35 days of age)

Oocyst burden in intestinal contents

Oocyst reduction (%)

Value (%)

p value

Value (%)

p value

Value (× 106, OPG)

p value

E. tenella

NSLC-PLGA

122.923 ± 22.402

0.6446

37.655 ± 7.341

<0.0001

0.889 ± 0.253

<0.0001

86.315

Blank

117.565 ± 28.329

–

21.599 ± 6.616

–

6.497 ± 1.345

–

–

E. necatrix

NSLC-PLGA

119.374 ± 26.856

0.8387

39.327 ± 7.097

<0.0001

0.882 ± 0.111

<0.0001

88.183

Blank

122.075 ± 31.445

–

22.545 ± 3.937

–

7.466 ± 1.457

–

–

E. maxima

NSLC-PLGA

124.445 ± 17.063

0.3584

39.882 ± 7.006

0.0012

1.990 ± 0.657

<0.0001

74.650

Blank

117.112 ± 17.726

–

26.566 ± 8.501

–

7.851 ± 0.842

–

–

E. acervulina

NSLC-PLGA

120.356 ± 13.725

0.6377

38.436 ± 8.171

0.0015

1.782 ± 0.592

<0.0001

79.078

Blank

123.103 ± 11.849

–

25.938 ± 6.671

–

8.518 ± 2.173

–

–

  1. Ten animals in each group were weighed at 14 (before the first immunization), 28 (before challenge), and 35 (seven days after challenge) days of age, and animals in each group were challenged with E. tenella at 28 days of age and euthanized seven days later. Intestinal contents were collected to evaluate the cross-protection of NSLC-PLGA nanospheres against four Eimeria spp. Each sample was assessed once, and values were estimated by the independent t test. Values are shown as the mean ± SD (n = 10). p values were obtained by comparison with the blank group.