Table 1 Vaccination strategies and challenge strategies.
Group | Vaccination strategies | Challenge strategies | |||
|---|---|---|---|---|---|
Treatment (each animal) | Vaccination time | Treatment (each animal) | Challenge time | Euthanasia time | |
Blank (PBS) | Equal volume of PBS | At 14 and 21Â days of age | Equal volume of PBS (0 oocysts) at 28Â days old | At 28Â days of age | At 35Â days of age, and the intestinal contents were separated |
Blank (Coccidia) | Equal volume of PBS | 5 × 104 oocysts of E. tenella for each animal at 28 days old | |||
Control | 200 μg of pET32a vector protein | ||||
NSLC | 200 μg of NSLC protein | ||||
NSLC-FA | NSLC-FA emulsions containing 200 μg of NSLC protein | ||||
NSLC-PLGA | NSLC-PLGA nanospheres containing 200 μg of NSLC protein | ||||
NSLC-CS | NSLC-CS nanospheres containing 200 μg of NSLC protein | ||||
NSLC-ISCOM | NSLC-ISCOM vaccines containing 200 μg of NSLC protein | ||||
NSLC-RG1 | NSLC-RG1 vaccines containing 200 μg of NSLC protein | ||||
NSLC-201VG | NSLC-201VG emulsions containing 200 μg of NSLC protein | ||||
NSLC-71VR | NSLC-71VR emulsions containing 200 μg of NSLC protein | ||||