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Figure 2 | Veterinary Research

Figure 2

From: A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens

Figure 2

Identification of the pET32a-NSLC vector and verification of the NSLC protein. A Map of the constructed pET32a-NSLC vector containing the NSLC protein sequence. B Double digestion analysis of the constructed pET32a-NSLC vector. Lane M: DL5000 marker; Lane 1: Double digestion with BamHI and SalI. C SDS–PAGE analysis of purified NSLC protein (Lane 1). Lane M: MW marker proteins. D Western blot analysis of NSLC protein. Purified NSLC protein was detected in the sera from E. tenella-infected chickens (Lane 1), E. acervulina-infected chickens (Lane 2), E. necatrix-infected chickens (Lane 3), E. maxima-infected chickens (Lane 4), and healthy chickens (Lane 5). Lane M: MW marker proteins.

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Veterinary Research

ISSN: 1297-9716