Table 1 Sequences and primer pair characteristics.
Primer | Plasmid | Primer sequence (5′ → 3′) | Accession No. |
|---|---|---|---|
P1 | pcaggs-IRF7-Myc | CATCATTTTGGCAAAGAATTCGCCACCATGGCAGCGGCGGAGAGCGAAG | MG707077.1 |
P2 | GGCAGAGGGAAAAAGATCTTCACAGGTCCTCCTCTGAGATCAGCTTCTGCTCGTCTATCTGCATGTTGTACTGCTCG | ||
P3 | pcaggs-RIG-I-Flag | CATCATTTTGGCAAAGAATTCGCCACCATGACGGCGGACGAGAAGCGG | MK636873.1 |
P4 | AAAAAGATCTGCTAGCTCGAGCTACTTATCGTCGTCATCCTTGTAATCGATCTTATCGTCGTCATCCTTGTAATCTCCCTTATCGTCGTCATCCTTGTAATCAAATGGTGGGTACAAGTT | ||
P5 | duck RIG-I (RT-PCR) | TCTCTGTCGGTCGGATAA | KC869660.1 |
P6 | TCATCAGGTTCTGCTTCTTC | ||
P7 | duck MDA5 (RT-PCR) | GCTGAAGAAGGCCTGGACAT | KJ451070.1 |
P8 | TCCTCTGGACACGCTGAATG | ||
P9 | duck MAVS (RT-PCR) | AGCCCAGAAATGAACCCCAG | KX290106.1 |
P10 | TCGAACTGCTGCTGGATGAG | ||
P11 | duck STING (RT-PCR) | CCACATCTTGATCCCGCTGA | XM_013100766.1 |
P12 | ATTGCGTAGAGGCTGTGCTT | ||
P13 | duck TBK1 (RT-PCR) | TGATCTATGAAGGTCGGCGTTT | MG772817.1 |
P14 | CTGCTCACTACGAAGATAGGATTCTC | ||
P15 | duck IRF7 (RT-PCR) | CGCCACCCGCCTGAAGAAGT | MG707077.1 |
P16 | CTGCCCGAAGCAGAGGAAGAT | ||
P17 | duck IFN-β (RT-PCR) | TCTACAGAGCCTTGCCTGCAT | KM035791.2 |
P18 | TGTCGGTGTCCAAAAGGATGT | ||
P19 | duck OASL (RT-PCR) | TCTTCCTCAGCTGCTTCTCC | KY775584.1 |
P20 | ACTTCGATGGACTCGCTGTT | ||
P21 | duck Mx (RT-PCR) | TGCTGTCCTTCATGACTTCG | NM_001310409.1 |
P22 | GCTTTGCTGAGCCGATTAAC | ||
P23 | UL41(RT-PCR) | TGATTTACACCGCTACCCTA | AFC61841.1 |
P24 | TCTCACTTCTTTCAGCCATT | ||
P25 | pGPU6/GFP/Neo-shIRF7 | GCTCATCGAGCAGTACAACAT | |
P26 | pGPU6/GFP/Neo-shNC | TTCTCCGAACGTGTCACGT |