Figure 1From: A multifunctional enolase mediates cytoadhesion and interaction with host plasminogen and fibronectin in Mycoplasma hyorhinisDetection of enolase on the surface of M. hyorhinis. A Flow cytometry analysis of the surface localization of enolase. Blank control, M. hyorhinis cells treated with PBS; Negative control, M. hyorhinis cells treated with preimmune serum; anti-rEno, M. hyorhinis cells treated with anti-rEno serum (A-1). The mean fluorescence intensity (MFI) of M. hyorhinis incubated with anti-rEno serum is expressed as the percentage of the corresponding strain incubated with preimmune serum. Results are expressed as mean ± standard deviation (SD) of three experiments with triplicate samples. Asterisks above charts indicate statistically significant differences (**p < 0.01; A-2). B Colony blot analysis of the surface localization of enolase. Immunostaining with anti-rEno serum (B-1) or preimmune serum (B-2) was performed after transferring M. hyorhinis colonies to a PVDF membrane.Back to article page