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Table 2 Overview of blood biochemistry.

From: Assessment of automated assays for serum amyloid A, haptoglobin (PIT54) and basic biochemistry in broiler breeders experimentally infected with Escherichia coli

Parameter

Infected (n = 14a)

Control (n = 15)

Prior to inoculation

2 dpi

4 dpi

6b dpi

Prior to inoculation

2 dpi

4 dpi

7 dpi

Total protein (g/L)

46.43 ± 3.79

45.95 ± 3.39

48.69 ± 3.37

45.80 ± 2.85

47.38 ± 3.03

49.81 ± 7.16

46.46 ± 1.68

48.82 ± 1.29

Albumin (g/L)

23.04 ± 1.53

22.83 ± 1.25

22.29 ± 1.61

21.94 ± 1.2

23.74 ± 1.75

24.91 ± 3.19

22.4 ± 1.0

23.16 ± 1.0

Alanine aminotransferase (U/L)

2.43 ± 1.65

1.8 ± 0.45

2.0 ± 1.23

2.25 ± 0.96

2.2 ± 1.15

2.6 ± 1.52

2.0 ± 1.0

3.2 ± 0.84

Alkaline phosphatase (U/L)

305.2 ± 69.90

327.8 ± 71.1

322.0 ± 118.4

335.4 ± 118.7

314.9 ± 76.26

321.6 ± 14.33

348.2 ± 60.12

264.6 ± 66.08

Total bilirubin (µmol/L)

3.93 ± 1.49*

2.8 ± 1.1*

4.8 ± 2.17*

5.75 ± 0.96*

5.47 ± 2.03

5.8 ± 1.92

7.0 + 1.4

8.6 ± 0.89

Bile acid (µmol/L)

26.71 ± 27.31

43.4 ± 13.94

36.8 ± 12.03

59.5 ± 30.16

14.0 ± 6.56

71.8 ± 36.29

34.6 ± 6.35

56.6 ± 34.59

Aspartate aminotransferase (U/L)

255.9 ± 48.72

307.6 ± 124.1

238.4 ± 55.02

301.5 ± 183.8

275.7 ± 46.33

297.4 ± 59.93

266.8 ± 22.08

227 ± 23.69

Creatinine (µmol/L)

11.5 ± 5.43

14.6 ± 3.05

10.8 ± 1.92

18.0 ± 8.83

8.87 ± 1.96

13.4 ± 4.72

9.6 ± 2.61

8.4 ± 3.36

Blood urea nitrogen (mmol/L)

1.84 ± 2.81

0.94 ± 2.1

2.16 ± 1.96

1.825 ± 3.39

3.02 ± 3.18

5.2 ± 3.33

4.04 ± 2.59

0.0 ± 0.0

Creatine kinase (U/L)

3711 ± 1540

5345 ± 4470

2918 ± 1615

4511 ± 673.3*

3895 ± 2117

4224 ± 1646

3234 ± 1905

2954 ± 971.4

Gamma-glutamyl transferase (U/L)

11.64 ± 3.52

14.2 ± 2.39

14.8 ± 6.42

10.0 ± 2.94

12.0 ± 3.89

13.4 ± 4.28

14.0 ± 4.74

13.2 ± 1.74

Calcium (mmol/L)

5.88 ± 0.56

4.82 ± 0.7*

5.38 ± 1.18

5.55 ± 1.2

5.86 ± 0.59

6.06 ± 0.57

6.33 ± 0.15

6.27 ± 0.15

Phosphate (mmol/L)

2.32 ± 0.36

2.08 ± 0.26

2.54 ± 0.52

2.55 ± 0.45

2.27 ± 0.35

2.46 ± 0.64

2.3 ± 0.07

2.18 ± 0.36

Magnesium (mmol/L)

1.71 ± 0.21

1.42 ± 0.22*

1.6 ± 0.34

1.57 ± 0.31

1.76 ± 0.23

1.93 ± 0.27

1.82 ± 0.7

1.87 ± 0.15

Serum amyloid A (mg/L)

0.14 ± 0.35

5.8 ± 7.7**

5.76 ± 11.88**

0.55 ± 1.1

0.26 ± 0.65

0.0 ± 0.0

0.0 ± 0.0

0.08 ± 0.18

Haptoglobin (PIT54) (g/L)

0.13 ± 0.03

0.17 ± 0.03

0.19 ± 0.03**c

0.14 ± 0.04

0.11 ± 0.013

0.14 ± 0.06

0.12 ± 0.013

0.16 ± 0.02

  1. All comparisons were made between the infected and control at corresponding days utilising an unpaired t-test for analysis of parametric data and a Mann–Whitney test for non-parametric data.
  2. dpi: days post-infection, n   number.
  3. aPreterm euthanasia due to animal welfare concerns.
  4. bAll infected birds were euthanised by the end of 6 dpi due to mild clinical signs (inappetence).
  5. cIt was only possible to collect a sufficient amount of heparin stabilised blood for haptoglobin (PIT54) analysis from four infected birds at 4 days post-infection (dpi).
  6. *p < 0.05, **p < 0.01.
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Veterinary Research

ISSN: 1297-9716