Figure 8

Tm, TsES, and TsKaSPI affect IPEC apoptosis. IPEC apoptosis was determined by staining with annexin V and PI followed by flow cytometry (A). The percentages of cells with different staining patterns are shown. The results presented are representative of three independent experiments (B). qPCR analysis of the relative expression of TLR2, TLR4, proapoptotic genes (Fas, Bax) and antiapoptotic genes (Bcl-xL, Bcl-2) in IPECs (C). Assays were performed in triplicate, and the data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the IPEC + PBS group, ⁺P < 0.05, ⁺⁺P < 0.01, ⁺⁺⁺P < 0.001 compared with the IPEC + Tm group, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 compared with the IPEC + TsES group.