Figure 10

Inhibition experiment to analyse the relationship between ERS and the NF-κB signalling pathway. Western blot analysis of the relative expression of Bip after the addition of NF-κB-specific inhibitors (A). ImageJ was used to analyse the grey values of the bands, and the results are shown in B. Western blot analysis of the relative expression of NF-κB and p-NF-κB after the addition of ERS-specific inhibitors (C). ImageJ was used to analyse the grey values of the bands, and the results are shown in D. The expression of proinflammatory cytokines was determined by ELISA, and the results are shown in E. The data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the IPEC + PBS group, ⁺P < 0.05, ⁺⁺P < 0.01, ⁺⁺⁺P < 0.001 for IPEC + TsKaSPI, and IPEC + PDTC + TsKaSPI group compared with the IPEC + PDTC group or IPEC + TsKaSPI, and IPEC + 4-PBA + TsKaSPI group compared with the IPEC + 4-PBA group, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 for IPEC + PDTC + TsKaSPI compared with the IPEC + TsKaSPI group or IPEC + 4-PBA + TsKaSPI compared with the IPEC + TsKaSPI group.