Table 1 Bacterial strains and plasmids used in this study

Plasmids

 pMC-Express

A broad host-range shuttle vector derived from pMIDG100, sodC promoter, Cm^r

[26]

 pCZb5

pMC-Express derivative with a 250 bp fragment containing the tpiA promoter

This work

 pRE112

sacB mobRP4 R6K ori Cm^r

[24]

 pCZ52

pRE112-ΔgatA^*

This work

 pCZ57

Insertion of complete gatA into pCZb5

This work

 pCZ58

Insertion of complete gatA and hptE into pCZb5

This work

Strains

 SM10 λ pir

E. coli thi thr-1 leu6 proA2 his-4 arg E2 lacY1 galK2, ara14xyl5 supE44, λpir

[25]

 PM0818

P. multocida 0818, Wild-type and virulent, LPS genotype L1

[23]

 PMZ2

PM0818 ΔgatA::kanR

This work