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Figure 4 | Veterinary Research

Figure 4

From: Immunogenicity and protection of a Pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks

Figure 4

Antibody responses and serum bactericidal assay. Ducks were immunized with two doses of 108 CFU of PMZ2 (ΔgatA*) orally or intranasally, given 2 weeks apart. PBS was also inoculated in the same way. Then, the levels of serum IgG (A), bile IgA (B) and tracheal IgA (C) in each group (n = 5) against the P. multocida antigens were measured by indirect ELISA at the indicated timepoints post-first immunization. Additionally, the P. multocida WT strain was treated with inactivated or active serum from day 21 and day 28 post-first immunization from each group for 5 h at 37 °C, and then the bacterial survival was calculated as the CFU after active serum treatment divided by the CFU after inactivated serum treatment (D).

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