Table 1 Composition of bovine enteroid monolayer (BEM) media.
From: Establishment of bovine 3D enteroid-derived 2D monolayers
Reagent | Concentration | Supplier |
|---|---|---|
Advanced DMEM/F12 | 1×  | ThermoFisher Scientific (TFS) |
l-glutamine | 2Â mM | TFS |
HEPES | 10Â mM | TFS |
Penicillin/Streptomycin | 50Â U/mL | TFS |
B27 supplement | 1×  | TFS |
N2 supplement | 1×  | TFS |
EGF (human) | 50Â ng/mL | TFS |
LDN 193189 | 100Â nM | Cambridge Bioscience |
R-Spondin 1 (human) | 100Â ng/mL | R&D Systems |
Noggin (human) | 50Â ng/mL | R&D Systems |
CHIR99021 | 10 µM | Stratech Scientific |
Y27632 | 10 µM | Cambridge BioScience |
SB202190 | 10 µM | Enzo Life Sciences |
LY2157299 | 500Â nM | Cambridge BioScience |
Nicotinamide | 10Â mM | Sigma-Aldrich |
FBS | 1% | GIBCO |