Figure 2
From: Establishment of bovine 3D enteroid-derived 2D monolayers
Formation of confluent 2D enteroids cultured in BEM media. Representative DIC images of 3-day-old 2D enteroids in BEM media supplemented with A 20% or B 1% FBS. Representative brighfield images of 10-day-old 2D enteroids in BEM media supplemented with C 20% or D 1% FBS. Representative brighfield images of 2D enteroids in BEM media without FBS on E day 3 and F day 4 of culture. Cells were seeded at 2.5 × 104 cells/well on Matrigel coated 96-well plate. Data is representative of three independent experiments from three individual calves.