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Figure 1 | Veterinary Research

Figure 1

From: Proteases secreted by Trichinella spiralis intestinal infective larvae damage the junctions of the intestinal epithelial cell monolayer and mediate larval invasion

Figure 1

Transwell experimental system used for identifying direct interactions between the main IIL ESP proteases and Caco-2 cells. Caco-2 cells were cultured on glass coverslips in a 12-well Transwell chamber, and 200 IIL pre-treated with various inhibitors were added to the insert in serum-free MEM medium and incubated at 37 ℃ for 2 h. The inhibitor doses used in the Transwell assay were 1.25 mM PMSF, 24 μM E-64, 2.4 mM 1,10-Phe and 10 μM pepstatin. There was a filter membrane on the inserted sieve to prevent IIL from invading and destroying the Caco-2 cell monolayer, but ESPs could pass through the insert and directly contact the monolayer, thus affecting epithelial TJ proteins.

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