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Table 1 Porcine specific primer sequences used for qPCR

From: Porcine reproductive and respiratory virus 2 infection of the fetus results in multi-organ cell cycle suppression

  Gene ID Symbol Forward primer Reverse primer Amplicon length Annealing Temp
Housekeeping 414,396 ACTB 5′-CCAGCACGATGAAGATCAAG-3′ 5′-AGTCCGCCTAGAAGCATTTG-3′ 171 60
396,581 HMBS 5′-AGGATGGGCAACTCTACCTG -3′ 5′-GATGGTGGCCTGCATAGTCT-3′ 83 61
397,637 PPIA 5′-CACTGCCAAGACTGAGTGGT-3′ 5′-TGTCCACAGTCAGCAATGGT-3′ 144 61
780,433 SDHA 5′-CTACAAGGGGCAGGTTCTGA-3′ 5′-AAGACAACGAGGTCCAGGAG-3′ 141 61
GOI 100,628,048 STX5 5′-TGCAGAGTCGTCAGAATGGA-3′ 5′-CCAGGATTGTCAGCTTCTCC-3′ 144 60
100,155,762 CDK1 5′-CAGCTCGCTACTCAACTCCA-3′ 5′-GAGTGCCCAAAGCTCTGAAA-3′ 135 61
100,154,715 CDK2 5′-CGGAGCTTGTTATCGCAAAT-3′ 5′-AGGGGTAGGGTTCACAAAGG-3′ 143 61
100,144,492 CDK4 5′-TGGTTACAAGTGGTGGGACA-3′ 5′-CCACAGAAGAGAGGCTTTCG-3′ 208 61
100,152,215 CDKN1A 5′-CATGTGGACCTGTTGCTGTC-3′ 5′-TTAGGGCTTCCTCTTGGAGA-3′ 168 61
110,259,225 CDKN1C 5′-ACGACACAGCGAACGAGAC-3′ 5′-GCAGCTCACGACTCAGCTC-3′ 164 60
100,525,560 CDKN2D 5′-TGCTGCTAGAGGAGGTCTGC-3′ 5′-CTGCCAAACATCATGACCTG-3′ 157 61
733,669 GADD45A 5′-ACGCCGCTCTCTCTCAGTAG-3′ 5′-CCCCACCTTATCCATCCTTT-3′ 98 59
100,513,507 PERP 5′-TGGTGGAAGTGTTCTCAGGA-3′ 5′-ACTCGCAGGAAGACAAGCAT-3′ 188 61
110,258,343 SIVA1 5′-CGCTACAGCTCAAGGTTCG-3′ 5′-GGGTGGTCTTCTCGAAAATCT-3′ 93 60