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Figure 7 | Veterinary Research

Figure 7

From: African swine fever virus MGF360-11L negatively regulates cGAS-STING-mediated inhibition of type I interferon production

Figure 7

The effect of siMGF360-11L on the expression of IFN-β, IL-1β and IL-6. PAMs were transfected with siMGF360-11L or nontargeting control siRNA and infected with ASFV at an MOI of 1.0 for 12 h or 24 h. A RT–PCR was conducted to measure IFN-β, ISG15, and ISG56 mRNA levels. B ELISA was carried out to measure the secretion of the cytokines IFN-β, IL-1β and IL-6 in the cell supernatant. C RT–PCR and Western blotting were performed to measure the expression level of P72. All experiments were independently repeated at least three times. The data are shown as the mean ± SD; n = 3. *p < 0.05, **p < 0.01, ***p < 0.001.

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