Figure 5
Influence of inhibitors on ASFV MGF360-11L the degradation of TBK1 and IRF7. HEK-293 T cells were co-transfected with IRF7 (200 ng, A) or TBK1 (200 ng, B) plasmids, Myc-GAPDH (100 ng), along with increasing doses of MGF360-11L (0, 100, 200, 400 ng) plasmids. 24 h post-transfection, Western blot analysis was performed with the indicated antibody. MGF360-11L (500 ng) and Myc-GAPDH (100 ng) plasmids were transfected with TBK1 (500 ng) and IRF7 (500 ng) plasmids into HEK-293 T cells, respectively. After 18 h of transfection, the cells were incubated with 3-MA (10 mM), NH4Cl (20 mM), MG132 (10 μM) and Z-VAD (20 μM) for 6 h. The cells were analyzed by Western blotting using the indicated antibody (C–F). All experiments were independently repeated at least three times. The data are shown as the mean ± SD; n = 3. *p < 0.05, **p < 0.01, ***p < 0.001.