Figure 4

ASFV MGF360-11L inhibited cGAS-STING-induced TBK1 and IRF3 phosphorylation. The MGF360-11L (1 μg) plasmid was co-transfected with cGAS (500 ng) and STING (500 ng) plasmids into HEK-293 T cells. At 24 h post-transfection, the cells were lysed with RIPA buffer, and the phosphorylation of IRF3 and TBK1 was measured by Western blotting. All experiments were independently repeated at least 3 times. The data are shown as the mean ± SD; n = 3. *p < 0.05, **p < 0.01, ***p < 0.001.