Figure 3

ASFV MGF360-11L could interact with TBK1 and IRF7. The MGF360-11L-HA (1 μg) plasmid was co-transfected with Flag-tagged IFN-I signaling molecule plasmids for cGAS (1 μg), STING (1 μg), TRAF3 (100 ng), TRAF6 (200 ng), TBK1 (1 μg), IKKi (500 ng), IRF3 (500 ng) and IRF7 (1 μg) into HEK-293 T cells, followed by IP with anti-Flag agarose affinity gel. The MGF360-11L-HA plasmid (1 μg) was co-transfected with Flag-tagged IFN-I signaling molecule plasmids for cGAS (1 μg), STING (1 μg), TRAF3 (100 ng), TBK1 (500 ng), IRF3 (200 ng) and IRF7 (1 μg) into HEK-293 T cells, followed by IP with anti-HA agarose affinity gel. Co-IP and Western blotting were carried out using anti-HA-HRP and anti-flag-HRP antibodies. All experiments were independently repeated at least three times. The data are shown as the mean ± SD; n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. IB, immunoblotting; HA, anti-HA-tagged monoclonal antibody; IP, immunoprecipitation; WCL, whole cell lysate.