Figure 3
From: Identification of a new amino acid mutation in the HN protein of NDV involved in pathogenicity
Syncytium formation and protein expression in BHK-21 monolayer cells coexpressing HN and F proteins for 24 h. A The extent of syncytium formation is shown for representative BHK-21 monolayer cells alone or cells expressing pCAGGS + F, HN-CE16 + F, HN-CI10 + F, or HN bearing the G215A and A430T substitutions. The cells were photographed under a fluorescence inverted microscope with 100-fold magnification, Bar = 100 µm. The fusion indices were determined by measuring the syncytium diameters (n = 10). B Total HN and F protein expression was verified by western blotting. The cleavage indices were determined according to the F1/F0 ratios, which were quantified by densitometry using ImageJ software. All marks indicate significance in comparison to HN-CE16 (100%), and the results are presented the mean ± SD of the results of three independent experiments. *p < 0.05, ***p < 0.001.