Pseudorabies virus (PRV) infection increased global protein synthesis in PK15 cells. Mock-infected PK15 cells and PK15 cells infected with PRV at an A MOI of 0.1 or B MOI of 1.0 were labelled with puromycin for 1 h at 5, 8, 11, and 23 hpi, and the cells were then harvested at 6, 9, 12, and 24 hpi. Cell lysates were subjected to Western blot analysis with the anti-puromycin mAb clone 12D10 to detect de novo protein synthesis. To monitor PRV replication, antibodies against PRV were used to detect PRV proteins. β-Actin was included in the Western blot analysis to document equivalent protein loading. The intensities of bands corresponding to puromycin-labelled proteins were determined by densitometry, and the protein synthesis rate is shown as the fold change after normalization to β-actin (bottom panels). The values are presented as the mean ± SD of triplicate experiments. * p < 0.05; ** p < 0.01; ***p < 0.001.