Table 3 Phenotypical and genotypical susceptibility profiles of five E. coli clones, each representing an outbreak.
From: Recurring outbreaks by the same Escherichia coli ST10 clone in a broiler unit during 18 months
Antimicrobial | Strain | DH68 | DH95 | DH119 4 | DH155 | DH162 | Resistance |
|---|---|---|---|---|---|---|---|
Case | 1 | 2 | 6 | 7 | Phenotype | ||
Amikacin | MIC (mg/L) | ≤ 4 | ≤ 4 | 8 | 8 | ≤ 4 | Sensitive |
Genotype | – | – | – | – | – | ||
Amoxicillin/clavulanic acid | MIC | 8 | > 8 | 8 | 8 | 8 | Sensitive |
Genotype | blaTEM-1B | blaTEM-1B | blaTEM-1B | blaTEM-1B | blaTEM-1B | ||
Ampicillin | MIC | > 8 | > 8 | > 8 | > 8 | > 8 | Resistant |
Genotype | blaTEM-1B | blaTEM-1B | blaTEM-1B | blaTEM-1B | blaTEM-1B | ||
Cefalexin | MIC | 4 | 8 | 4 | 4 | 4 | Sensitive |
Genotype | – | – | – | – | – | ||
Cefazolin | MIC | 4 | 4 | 4 | 4 | 4 | Intermediate |
Genotype | – | – | – | – | – | ||
Cefovecin | MIC | 0.5 | 1 | 0.25 | 0.25 | 0.5 | Sensitive |
Genotype | – | – | – | – | – | ||
Cefpodoxime | MIC | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | Sensitive |
Genotype | – | – | – | – | – | ||
Ceftazidime | MIC | ≤ 4 | ≤ 4 | ≤ 4 | ≤ 4 | ≤ 4 | Sensitive |
Genotype | – | – | – | – | – | ||
Chloramphenicol | MIC | 4 | 32 | 8 | 4 | 4 | Sensitive/resistant |
Genotype | – | floR | – | – | – | ||
Doxycycline | MIC | > 8 | > 8 | > 8 | > 8 | > 8 | Resistant |
Genotype | tetA | tetA | tetA | tetA | tetA | ||
Enrofloxacin | MIC | ≤ 0.12 | ≤ 0.12 | ≤ 0.12 | ≤ 0.12 | ≤ 0.12 | Sensitive |
Genotype | – | – | – | – | – | ||
Gentamicin | MIC | 1 | 1 | 1 | 1 | 1 | Sensitive |
Genotype | – | – | – | – | – | ||
Imipenem | MIC | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | Sensitive |
Genotype | – | – | – | – | – | ||
Marbofloxacin | MIC | ≤ 0.12 | ≤ 0.12 | ≤ 0.12 | ≤ 0.12 | ≤ 0.12 | Sensitive |
Genotype | – | – | – | – | – | ||
Orbifloxacin | MIC | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | ≤ 1 | Sensitive |
Genotype | – | – | – | – | – | ||
Piperacillin/tazobactam | MIC | ≤ 8 | ≤ 8 | ≤ 8 | ≤ 8 | ≤ 8 | Sensitive |
Genotype | blaTEM-1B | blaTEM-1B | blaTEM-1B | blaTEM-1B | blaTEM-1B | ||
Pradofloxacin | MIC | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 | ≤ 0.25 | Sensitive |
Genotype | – | – | – | – | – | ||
Tetracycline | MIC | > 16 | > 16 | > 16 | > 16 | > 16 | Resistant |
Genotype | tetA | tetA | tetA | tetA | tetA | ||
Trimethoprim/sulfamethoxazole | MIC | > 4 | > 4 | > 4 | > 4 | > 4 | Resistant |
Genotype | dfrA1/sul2 | dfrA1/sul2 | dfrA1/sul2 | dfrA1/sul2 | dfrA1/sul2 |