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Figure 7 | Veterinary Research

Figure 7

From: Prototheca spp. induce an inflammatory response via mtROS-mediated activation of NF-κB and NLRP3 inflammasome pathways in bovine mammary epithelial cell cultures

Figure 7

Expression IL-1β protein in bMECs. A Treatment with MT (10 µM) for 12 h inhibited IL-1β expression in P. bovis- or P. ciferrii-infected bMECs. Red fluorescence is expression of IL-1β protein. B–D Treatment with MT (10 µM) for 12 h inhibited expression of Pro IL-1β and IL-1β proteins in P. bovis- or P. ciferrii-infected bMECs. “-” and “ + ” after MT indicated that MT was not or were added, respectively; “-” and “ + ” after P. bovis indicated that P. bovis was not or were added, respectively; “-” and “ + ” after P. ciferrii indicated that P. ciferrii was not or were added, respectively. Data represent means ± SD of 3 independent experiments. *P < 0.05 or **P < 0.01, difference compared to the control; #P < 0.05 or ##P < 0.01, difference compared to the P. ciferrii infection group; &P < 0.05 or &&P < 0.01, difference compared to the P. bovis infection group.

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