Figure 6

Expression of NLRP3 inflammasome pathway proteins in bMECs. A and B in P. bovis- or P. ciferrii-infected bMECs. Treatment with MT (10 µM) for 12 h inhibited protein expression of NLRP3 and ASC in P. bovis- or P. ciferrii-infected bMECs. Green fluorescence is expression of NLRP3 and ASC proteins. C–G Treatment with MT (10 µM) for 12 h inhibited expressions of NLRP3, Pro Caspase1, Caspase1 p20, and ASC proteins in P. bovis- or P. ciferrii-infected bMECs. “-” and “ + ” after MT indicated that MT was not or were added, respectively; “-” and “ + ” after P. bovis indicated that P. bovis was not or were added, respectively; “-” and “ + ” after P. ciferrii indicated that P. ciferrii was not or were added, respectively. Data represent means ± SD of 3 independent experiments. ^*P < 0.05 or ^**P < 0.01, difference compared to the control; ^#P < 0.05 or ^##P < 0.01, difference compared to the P. ciferrii infection group; ^&P < 0.05 or ^&&P < 0.01, difference compared to the P. bovis infection group.