Figure 5

Expression of NF-κB pathway proteins in bMECs. A and B Green fluorescence is expression of NF-κB p65 and p-NF-κB p65 proteins; treatment with MT (10 µM) for 12 h inhibited expression of these proteins in bMECs infected with P. bovis or P. ciferrii. C–G Treatment with MT (10 µM) for 12 h reduced expression levels of NF-κB p65, p-NF-κB p65, IκBα, and p-IκBα proteins in bMECs infected with P. bovis or P. ciferrii. “-” and “ + ” after MT indicated that MT was not or was added, respectively; “-” and “ + ” after P. bovis indicated that P. bovis was not or were added, respectively; “-” and “ + ” after P. ciferrii indicated that P. ciferrii was not or were added, respectively. Data represent means ± SD of 3 independent experiments. ^*P < 0.05 or ^**P < 0.01, difference compared to the control; ^#P < 0.05 or ^##P < 0.01, difference compared to the P. ciferrii infection group; ^&P < 0.05 or ^&&P < 0.01, difference compared to the P. bovis infection group.